[Bacterial colonization in areas of burnt skin and healthy children younger than 15 years, seen COANIQUEM, Santiago, Chile]. / Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile.

UNLABELLED: Bacterial colonization in patients with burns skin without infection and outpatient management has been poorly studied. OBJECTIVE: To quantify and identify the type of bacteria that colonize healthy body areas homologous location of the skin in children from 1 to 15 years, seen COANIQUEM, Santiago, Chile. Per patient, we studied 204 samples from each zone. The isolated microorganisms were S. epidermidis 35, 3%; others Staphylococcus coagulase negative, 25.8%; S. aureus, 4.4%; and S. viridans group 1.4%. In the subgroup of children who had a count between 1 and 1000 cfu, there was a higher proportion of cfu in burned skin healthy skin (p = 0.0359). The association between depth of the lesion and the bacterial count obtained p = 0.034. CONCLUSION: In outpatient treatment, the microorganisms are expected in healthy skin, burned skin is evident in lower counts probably associated with epithelial damage.

Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile / Bacterial colonization in areas of burnt skin and healthy children younger than 15 years, seen COANIQUEM, Santiago, Chile

Bacterial colonization in patients with burns skin without infection and outpatient management has been poorly studied. Objective: To quantify and identify the type of bacteria that colonize healthy body areas homologous location of the skin in children from 1 to 15 years, seen COANIQUEM, Santiago, Chile. Per patient, we studied 204 samples from each zone. The isolated microorganisms were S. epidermidis 35, 3%; others Staphylococcus coagulase negative, 25.8%; S. aureus, 4.4%; and S. viridans group 1.4%. In the subgroup of children who had a count between 1 and 1000 cfu, there was a higher proportion of cfu in burned skin healthy skin (p = 0.0359). The association between depth of the lesion and the bacterial count obtained p = 0.034. Conclusion: In outpatient treatment, the microorganisms are expected in healthy skin, burned skin is evident in lower counts probably associated with epithelial damage.

La colonización bacteriana de la piel de pacientes quemados, sin infección y de manejo ambulatorio, ha sido escasamente estudiada. Objetivo: Cuantificar e identificar el tipo de bacterias que colonizan las zonas cruentas y las zonas sanas de localización homóloga de la piel, en niños entre uno y 15 años de edad que son tratados de forma ambulatoria en la Corporación de Ayuda al Niño Quemado (COANIQUEM) en Santiago de Chile. Pacientes y Métodos: Se estudiaron 204 pacientes quemados en los que se tomó una muestra para cultivo desde la zona de la quemadura y otra desde el sitio homólogo sin quemadura. Resultados: Las especies bacterianas aisladas desde ambos tipos de muestra fueron similares: Staphylococcus epidermidis; otras especies de Staphylococcus coagulasa negativa; Staphylococcus aureus, y Streptococcus grupo viridans. El recuento bacteriano de uno a 999 ufc/ 10,5 cm² fue significativamente superior en las muestras de piel sana (p = 0,0359). La asociación entre mayor profundidad de la lesión y el menor recuento bacteriano obtuvo un p = 0,034. Conclusión: En niños quemados de entre uno y 15 años, de manejo ambulatorio, los microorganismos que colonizan la zona quemada fueron los mismos que colonizan la piel sana, pero en recuento inferior.

[Acute gastroenteritis caused by a Vibrio cholerae non-O1, non-O139 strain harboring a genetic region homologous to the VpaI-7 pathogenicity island]. / Gastroenteritis aguda causada por Vibrio cholerae no-O1, no-O139 que porta una región homologa a la isla de patogenicidad VpaI-7.

Pathogenic Vibrio cholerae isolates, the etiologic agents of cholera, generally express one of two O antigens (O1 or O139). Most environmental isolates are nonpathogenic and are referred to as "non-O1, non-O139". However some V. cholerae non-O1, non-O139 strains are clearly pathogenic and have caused outbreaks or sporadic cases of gastroenteritis and extraintestinal infections in humans. We report a case of acute gastroenteritis by a V. cholerae non-O1, non-O139 harboring a genetic region homologous to a segment of the VpaI-7 V. parahaemolyticus pathogenicity island.

Gastroenteritis aguda causada por Vibrio cholerae no-O1, no-O139 que porta una región homologa a la isla de patogenicidad VpaI-7 / Acute gastroenteritis caused by a Vibrio cholerae non-O1, non-O139 strain harboring a genetic region homologous to the VpaI-7 pathogenicity island

Pathogenic Vibrio cholerae isolates, the etiologic agents of cholera, generally express one of two O antigens (O1 or O139). Most environmental isolates are nonpathogenic and are referred to as "non-O1, non-O139". However some V. cholerae non-O1, non-O139 strains are clearly pathogenic and have caused outbreaks or sporadic cases of gastroenteritis and extraintestinal infections in humans. We report a case of acute gastroenteritis by a V. cholerae non-O1, non-O139 harboring a genetic region homologous to a segment of the VpaI-7 V. parahaemolyticus pathogenicity island.

Cepas patogénicas de Vibrio cholerae, el agente causal del cólera, expresan generalmente uno de dos antígenos O (denominados O1 u O139). La mayoría de las cepas ambientales son no patogénicas y corresponden al tipo denominado "no-O1, no-O139". Sin embargo, algunas cepas de este tipo son claramente patogénas y han causado brotes de gastroenteritis e infecciones extra-intestinales en humanos. Se reporta un caso clínico de gastroenteritis aguda causado por una cepa de V. cholerae no-O1, no-O139 que contiene en su genoma una región homóloga a un segmento de la isla de patogenicidad VpaI-7 descrita previamente en V. parahaemolyticus.

[Non-toxigenic hemolytic Vibrio cholerae non-O1 non-O139 fatal septicemia. Report of one case]. / Septicemia fatal causada por Vibrio cholerae no-O1, no-O139 hemolítico en Chile: Caso clínico.

We report a 70-year-old woman, who had recently consumed shellfish, that was admitted to the intensive care unit with septic shock and died 19 hours later due to a multi-organic failure. Microbiological, serological and molecular assays confirmed a hemolytic tdh+ Vibrio cholerae non-01, non 0139 as the etiologic agent.

Septicemia fatal causada por Vibrio cholerae no-O1, no-O139 hemolítico en Chile: Caso clínico / Non-toxigenic hemolytic Vibrio cholerae non-O1non-O139 fatal septicemia: Report of one case

We report a 70-year-old woman, who had recently consumed shellfish, that was admitted to the intensive care unit with septic shock and died 19 hours later due to a multi-organic failure. Microbiological, serological and molecular assays confirmed a hemolytic tdh+ Vibrio cholerae non-01, non 0139 as the etiologic agent (Rev Méd Chile 2009; 137: 1193-6).

[In vitro activity of telithromycin against community acquired respiratory pathogens]. / Estudio de la actividad comparativa in vitro de telitromicina en patógenos respiratorios adquiridos en la comunidad en 13 centros clínicos chilenos.

BACKGROUND: Telithromycin is a new ketolide antimicrobial, that can be useful for the treatment of respiratory infections. AIM: To compare in vitro activity of telithromycin against respiratory pathogens, isolated in outpatient clinics. MATERIAL AND METHODS: Two hundred eighty strains isolated from patients with respiratory infections, were studied. The strains studied were S pneumoniae, penicillin sensitive (SPNS:57); intermediate (SPNI:35), resistant (SPNR:25); S pyogenes (SP:57); H influenzae (HIN 51); M catarrhalis (MC:25) and S aureus meticillin sensitive (SAUS:30). Minimal inhibitory concentration (MIC) by broth microdilution was studied for telitrhomycin and levofloxacin in all strains. Other antimicrobials studied, but not in all strains were erythromycin, clindamycin, trimetoprim sulphamethoxazole, oxacillin, amoxicillin-clavulanic acid and cefuroxime. RESULTS: All strains were sensible to telithromycin at a concentration -4 microg/ml. MIC 90 and its range for SPNS was 0.03 microg/ml (-0.004-0.12), for SPNI was 0.03 microg/ml (-0.004-025), for SPNR was 0.06 microg/ml (-0.004-0.25), for HIN was 2 microg/ml (0.12-4), for SP was 0.5 microg/ml (-0.004-2), for MC was 0.5 microg/ml (0.06-2) and for SAU was 0.25 microg/ml (0.06-0.25). CONCLUSIONS: All studied pathogens were sensible to telithromycin in vitro. This antimicrobial is an alternative for the treatment of community acquired respiratory infections.

Estudio de la actividad comparativa in vitro de telitromicina en patógenos respiratorios adquiridos en la comunidad en 13 centros clínicos chilenos / In vitro activity of telithromycin against community acquired respiratory pathogens

Background: Telithromycin is a new ketolide antimicrobial, that can be useful for the treatment of respiratory infections. Aim: To compare in vitro activity of telithromycin against respiratory pathogens, isolated in outpatient clinics. Material and methods: Two hundred eighty strains isolated from patients with respiratory infections, were studied. The strains studied were S pneumoniae, penicillin sensitive (SPNS:57); intermediate (SPNI:35), resistant (SPNR:25); S pyogenes (SP:57); H influenzae (HIN 51); M catarrhalis (MC:25) and S aureus meticillin sensitive (SAUS:30). Minimal inhibitory concentration (MIC) by broth microdilution was studied for telitrhomycin and levofloxacin in all strains. Other antimicrobials studied, but not in all strains were erythromycin, clindamycin, trimetoprim sulphamethoxazole, oxacillin, amoxicillin-clavulanic acid and cefuroxime. Results: All strains were sensible to telithromycin at a concentration ¡4 µg/ml. MIC 90 and its range for SPNS was 0.03 µg/ml (¡0.004-0.12), for SPNI was 0.03 µg/ml (¡0.004-025), for SPNR was 0.06 µg/ml (¡0.004-0.25), for HIN was 2 µg/ml (0.12-4), for SP was 0.5 µg/ml (¡0.004-2), for MC was 0.5 µg/ml (0.06-2) and for SAU was 0.25 µg/ml (0.06-0.25). Conclusions: All studied pathogens were sensible to telithromycin in vitro. This antimicrobial is an alternative for the treatment of community acquired respiratory infections.